Ligate

To tie or bind something, especially a blood vessel or duct, to stop the flow of fluid or substance. In biological contexts, this can refer to the joining of DNA fragments. The process often involves the use of sutures, clips, or specialized enzymes. Ligation is a critical technique in surgery, molecular biology, and chemistry, ensuring controlled manipulation and closure of anatomical structures or molecular components. It's essential for preventing leaks, controlling reactions, and creating stable connections.

Ligate meaning with examples

• During the surgery, the surgeon had to ligate the bleeding artery to prevent excessive blood loss. The process involved carefully tying off the vessel with a strong suture. This quick and precise action was vital for stabilizing the patient and ensuring a successful outcome. The meticulous ligation of the vessel stopped any further bleeding.
• In molecular biology, scientists used ligase enzymes to ligate two separate DNA fragments, creating a new, combined molecule. This was essential for cloning genes and creating recombinant DNA. The effective ligation allowed them to study and manipulate genetic information. The use of this technique made their research more efficient and precise.
• After the chemical reaction, it was necessary to ligate the two reactants to produce the desired product. This step involved a specific binding agent and precise temperature control. By carefully ligating the molecules, the chemists were able to synthesize the compound successfully. The entire process required them to pay very close attention to detail.
• The damaged bile duct required immediate repair, involving the careful ligation of the surrounding tissue. The surgeon utilized micro-surgical techniques to ensure a tight and secure closure. Without properly ligating this area, the patient could develop a life-threatening infection. Skill and precision were critical in this situation.
• As part of his restoration, the conservator had to ligate the delicate pieces of the artifact together with a non-damaging adhesive. This required immense patience and meticulous precision. He needed to ensure that the fragments of the relic would reassemble together without destroying their beauty. The success of the restoration depended on his ligation.